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Toxicology of Cinnamomum:

Since 1986, there have been an ethical and legal obligations imposed on countries that have signed the convention of council of Europe (European Council 1986) in view to replace, reduce and refine the use of animals in clinical research. Since then, several research have been carried out on water flees such as daphnia.  Based on the preclinical and the data available on the different composition of cinnamon, a lethal dose test was carried out in order to determine the toxicity of cinnamon. This LD50 test was carried out in the aim of:-

1.      Determining pre clinically the best dose to be administered to patients in the clinical trials

2.      Assessing any potential risk in the taking of cinnamomum

3.      Having an approach to the 3R theory in the aim to reduce the use of animals in pre clinical research

 

Equipments:-

Cinnamomum, 5 boiling tubes, 5 beakers, a micro scale, Daphnia flees, Petri dish

Methods:-

Cinnamomum was prepared in five different concentrations of 1µg/150ml, 2µg/150ml, 4µg/150ml, 8µg/150ml, 16µg/150ml respectively. The cinnamomum was first measured very accurately with a decimal error of +0.05µg on a micro scale. The different weights of cinnamomum were each boiled in 10ml of water for infusion. Each concentration was then transferred in a beaker and left to cool down for 15minutes. the daphnia sample were then poured and the volume of water was round up to 150ml each. The sample was then tranferred in a Petri dish for counting. The reaction was then recorded after three hours in table 2:-

 

Table 2: the lethal dose test determination table

Beaker

Concentration

Alive

Dead

Lethal dose %

  1.  

1µg/150ml

All

None

0

  1.  

2µg/150ml

All

None

0

  1.  

4µg/150ml

48

46

49

  1.  

8µg/150ml

31

62

67

  1.  

16µg/150ml

None

All

100

The LD50 curve based on the above table is as follows:

 


Figure 1 Lethal dose test (LD50)

 

From this experiment, it can be deduced that the LD50 is 4µg/150ml having a percentage nearer to 50%. Therefore, from this experiment, the dosage to be administered to patients will go on a clinical trials at this concentration.


 
 

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